Protein synthesis is essential for every aspect of life. As result, the disruption and abnormalities of translation are linked to numerous diseases including cancer, viral infections, and metabolic and developmental diseases. We are interested in 1) how mRNAs are selected by translational system, and how it leads to the progression of the disease. 2) How molecular machine called ribosome makes proteins with astonishing speed and precision, reading 20 codons per second while making less than 1 mistake in 3000 codons, virtually insuring that no error is ever made. We use combination of biochemistry and the super resolution fluorescent microscopy to answer these questions.